The DNA synthesizing subunit of polymerase-primase from calf thymus.
نویسندگان
چکیده
منابع مشابه
DNA polymerase lambda from calf thymus preferentially replicates damaged DNA.
A new gene (POLL), has been identified encoding the novel DNA polymerase lambda and mapped to mouse chromosome 19 and at human chromosome 10. DNA polymerase lambda contains all the critical residues involved in DNA binding, nucleotide binding, nucleotide selection, and catalysis of DNA polymerization and has been assigned to family X based on sequence homology with polymerase beta, lambda, mu, ...
متن کاملCalf thymus polymerase.
The term polymerase has been applied to the deoxyribonucleoside triphosphate-polymerizing enzyme isolated from Escherichia coli (1). Enzymes with similar activity have been purified from regenerating rat liver (2) and calf thymus gland (3). An ample and varied supply of such polymerases is desirable for studies on the mechanism of deoxyribonucleic acid synthesis. The present work describes a pa...
متن کاملApparent stimulation of calf thymus DNA polymerase alpha by ATP.
The mechanism by which millimolar concentrations of ATP stimulate the activity and increase the processivity of calf thymus DNA polymerase alpha has been investigated with poly(dA)/oligo(dT) as template/primer to eliminate possible effects due to primer synthesis. The effect of ATP on the rate of DNA synthesis with this template/primer was found to be dependent upon whether or not the ATP was n...
متن کاملFurther studies on partially purified calf thymus DNA polymerase a.
Attempts to prevent the urea conversion of a 200-230,000 molecular weight DNA polymerase alpha to a 150-170,000 molecular weight form by the inclusion of protease inhibitors have not been successful. No other method has been found capable of dissociating a 50-70,000 fragment or subunit from the DNA polymerase subunit. Addition of this 50-70,000 subunit to the polymerase subunit does not aid the...
متن کاملFour different DNA helicases from calf thymus.
Using a strand displacement assay we have followed DNA helicase activities during the simultaneous isolation of several enzymes from calf thymus such as DNA polymerases alpha, delta, and epsilon, proliferating cell nuclear antigen, and replication factor A. Thus we were able to discriminate and isolate four different DNA helicases called A, B, C, and D. DNA helicase A is identical with the enzy...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1992
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)50673-1